O081 Investigating the FLYWCH1/ β-catenin signalling in pancreatic cancer

Abstract Introduction β-catenin is a protein molecule of the E-cadherin/catenin adhesion complex that maintains epithelial cell-cell and intracellular signalling. The mutation overexpression leads to cancer, including pancreatic ductal adenocarcinoma (PDAC). FLYWCH1 newly identified with zinc-finger type DNA-binding domain interacts nuclear β-catenin, leading increased cell-matrix in cancer stem cells. This study aimed explore roles crosstalk between Wnt/β-catenin signalling for first time Methods We performed Western Blot analysis extraction FLYWCH1/β-catenin, E-cadherin GSK3-b AsPc-1, PANC-1, MIA PaCa-2 cell lines. Recommended concentrations antibodies were used detection proteins interest. Tubulin was as loading control. Fluorescent used, density detected measured. Results Our results demonstrate evidence differential expression PANC-1 had less FLYWCH1, but moderate AsPC-1. E-cadherin, β catenin, also expressed. These data suggest levels correlated unphosphorylated suppress transcriptional product via controlling aberrant pathway while promoted attachment. Conclusion Wnt1/β-catenin activity negatively Thus, could have tumour suppressive activities. Future studies are required elucidate their therapeutic biomarkers efficacy. Take-home message control B-catenin which suppression. would be target treatment future.